|
週次 |
日期 |
單元主題 |
|
第1週 |
|
Lec: Introduction
Lab: Whole mount & Clearing technique |
|
第2週 |
|
Lec: Tissue fixation & processing
Lab: Free-hand section & Vibratome |
|
第3週 |
|
Lec: Paraffine sectioning
Lab: Paraffine sectioning
(Tissue fixation and processing) |
|
第4週 |
|
Lec: Tissue embedding
Lab: Tissue embedding |
|
第5週 |
|
Lec: Sectioning
Lab: Coating slides & Sectioning |
|
第6週 |
|
Lec: Wood section & Maceration
Lab: Wood section |
|
第7週 |
|
Lec: Stains
Lab: Maceration |
|
第8週 |
|
Lec: Staining
Lab: Section staining |
|
第9週 |
|
Lec: Light microscopy
Lab: Light microscopy
(Fluorescence; Nomarski; Phase microscopy) |
|
第10週 |
|
Lec: Midterm Exam.
Lab: General discussion |
|
第11週 |
|
Lec: Image processing
Lab: Website search for biological microtechniques |
|
第12週 |
|
Lec: Image interpretation
Lab: Identification of cellular & subcellular structures – artifacts and pitfalls |
|
第13週 |
|
Lec: Presentation of microscopic data
Lab: Individual assistance to student projects |
|
第14週 |
|
Lec: Immunocytochemistry
Lab: Immunocytochemistry |
|
第15週 |
|
Lec: In situ hybridization
Lab: In situ hybridization |
|
第16週 |
|
Lec & Lab: Distribution of final, take-home exam. |